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2 edition of Developing new matrices for matrix-assisted laser desorption/ionisation mass spectrometry found in the catalog.

Developing new matrices for matrix-assisted laser desorption/ionisation mass spectrometry

Nicholas Carl Charnley

Developing new matrices for matrix-assisted laser desorption/ionisation mass spectrometry

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  • 7 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 2000.

StatementNicholas Carl Charnley.
The Physical Object
Paginationxxxii, 278p.
Number of Pages278
ID Numbers
Open LibraryOL21004152M

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is used for the determination of molecular weights of different chemical compounds. We describe here the use of MALDI-TOF mass spectrometry to detect a carbapenem antibiotic, meropenem, and its degradation products. Buffered meropenem solution ( mM Tris-HCl, pH ) was mixed with an overnight culture. Return to "Matrix-assisted laser desorption/ionization" page. Last edited on 1 July , at Content is available under CC BY-SA unless otherwise noted.


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Developing new matrices for matrix-assisted laser desorption/ionisation mass spectrometry by Nicholas Carl Charnley Download PDF EPUB FB2

Matrix-assisted laser desorption/ionization (MALDI) is one of the most successful “soft” ionization methods in the field of mass spectrometry and enables the analysis of a broad range of molecules, including lipids. Although the details of the ionization process are still unknown, the importance of the matrix is commonly accepted.

Both, the development of and the search for useful Cited by:   1. Introduction. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a modification of laser desorption/ ionization mass spectrometry (LDI-MS that allows for the analysis and detection of intact high molecular weight : Shelley N.

Jackson, Amina S. Woods. In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.

It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other. This work involves the investigation of a group of uniform materials based on organic salts (GUMBOS) as matrices for matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) for analysis of hydrophobic biomolecules.

GUMBOS are new class of materials that show great promise as MALDI matrices because they are able to form a thin-layer on the MS target, thereby Author: Al Ghafly, Hashim Abdullah.

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other Author: David J Strumfels.

Matrix-assisted laser desorption-ionization mass spectrometry (MALDI–MS) is a practically effective tool for the measurement of natural and synthetic polymers.

The ability to ionize a broad range of materials, high sensitivity, broad mass range, fast sample preparation, and the absence of fragmentation are principal characteristics of the. Matrix-assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Oligosaccharides in Soybean and Bean Leaf with Ionic Liquid as Matrix.

Chinese Journal of Analytical Chemistry45 (8), DOI: /S(17) The discovery of new matrices that are suitable for in situ analysis of low molecular-weight compounds by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important technological aspect of tissue imaging.

In this work, ten natural flavonoid compounds, including flavone and nine of its mono- or polyhydroxyl-substituted analogues (3-hydroxyflavone, 5-hydroxyflavone. Analyte incorporation and ionization in matrixassisted laser desorption/ionization visualized by pH indicator molecular probes.

Anal. Chem.73, – Giebel R, Worden C, Rust SM. Microbial fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) applications and challenges.

In recent years there has been considerable interest in developing matrix‐free laser desorption/ionization by using nanostructured substrates to substitute the conventional organic matrices, which is often referred as surface‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (SALDI‐TOF MS).

Ionic matrices pre-spotted matrix-assisted laser desorption/ionization plates for patient maker following in course of treatment, drug titration, and MALDI mass spectrometry imaging. Analytical Biochemistry(1),   Recent research has focused on increasing the evidentiary value of latent fingerprints through chemical analysis.

Although researchers have optimized the use of organic and metal matrices for matrix‐assisted laser desorption/ionization‐mass spectrometry imaging (MALDI‐MSI) of latent fingerprints, the use of development powders as matrices has not been fully investigated.

Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a robust tool for spatially resolved analysis of biomolecules in situ.

Recent advances in high ionization-efficiency MALDI matrices, new matrix deposition procedures, and the development of high spatial-resolution a. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has become an important technique for the analysis of biological macromolecules.

In particular, the identification of microorganisms is a hotspot in recent research. We have designed and assembled a MALDI-TOF MS for biological applications. Masoud Zabet‐Moghaddam, Ralf Krüger, Elmar Heinzle, Andreas Tholey, Matrix‐assisted laser desorption/ionization mass spectrometry for the characterization of ionic liquids and the analysis of amino acids, peptides and proteins in ionic liquids, Journal of Mass Spectrometry, /jms, 39, 12, (), ().

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry. MALDI mass spectrometry imaging (MSI) is a label-free method for the multiplexed analysis of biomolecules in their tissue morphological context and therefore closes one of the last remaining open gaps in tissue-based research.

From: Advances in Cancer Research, Related terms. This chapter describes the application of two-dimensional gel electrophoresis (2DGE) combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in the analysis of rat eye lens proteins.

The main purpose was to identify proteins that may serve as potential biomarkers in age-related cataract formation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an emerging analytical tool for detecting and identifying microorganisms.

It offers high sensitivity, simple sample preparation processes, low sample consumption volumes, and the possibility of automated and high-throughput analyses. Matrix-Assisted Laser Desorption/Ionisation mass spectrometry imaging (MALDI-MSI) has the potential to provide new insights into the molecular analysis of plants by providing high spatial resolution information about proteins and potentially quantitative changes during plant development or those induced by environmental variation.

A matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) based approach was developed for the rapid analyses of cellular glycerophospholipids.

Through multiplexed solvent-enabled optimization of analyte-matrix interactions during the crystallization process, over a fold increase in S/N was achieved using. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is commonly used for the characterization of synthetic or natural polymers.

9 Information such as average molecular weights (weight and number; M w and M n), nature and number of repeating units, and end-group determination can be deduced form MALDI-TOF. Improving the resolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by exploiting the correlation between ion position and velocity.

Rapid Communications in Mass Spectrometry8 (11), We combined targeted metabolite profiling with matrix-assisted laser desorption/ionization-mass spectrometry imaging to investigate accumulation of dhurrin, its recycling products and key general metabolites in four different sorghum lines during 72 h of grain imbibition, germination and early seedling development, as well as the spatial.

Recent research has focused on increasing the evidentiary value of latent fingerprints through chemical analysis. While researchers have optimized the use of organic and metal matrices for matrix‐assisted laser desorption/ionization ‐ mass spectrometry imaging (MALDI‐MSI) of latent fingerprints, the use of development powders as matrices has not been fully investigated.

Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared. Rapid Commun Mass Spectrom.

Dec; 6 (12)– Nordhoff E, Cramer R, Karas M, Hillenkamp F, Kirpekar F, Kristiansen K, Roepstorff P. Ion stability of nucleic acids in infrared matrix-assisted laser desorption.

Sublimation as a method of matrix application for mass spectrometric imaging. Journal of the American Society for Mass Spectrometry, 18(9), Fitzgerald, M. C., Parr, G. R., & Smith, L.

Basic matrixes for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides. Application of nonpolar matrices for the analysis of low molecular weight nonpolar synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Journal of the American Society for Mass Spectrometry11 (8), A Rapid Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Method for Single-Plasmid Tracking in an Outbreak of Carbapenem-Resistant Enterobacteriaceae.

Journal of Clinical Microbiology. 52(8): – Dreisewerd, K. Recent methodological advances in MALDI mass spectrometry. It has been challenging to detect small analytes in both positive and negative ion modes using organic matrices in conventional matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).

Herein, TiO2 nanowires are presented as a solid matrix to form dual ions of. Clinical impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the management of inpatient pneumonia without additional antimicrobial stewardship support Ann Marie Porreca Kratz (a1), Kaede V.

Sullivan (a2) and Jason C. Gallagher (a3). Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow. A bacterial or fungal colony (typically single) is “picked” from a culture plate to a “spot” on a MALDI-TOF MS target plate (a reusable or disposable plate with a number of test spots) using a wooden or plastic stick, pipette tip, or loop.

The ability to charge huge biomolecules without breaking them apart has made matrix-assisted laser desorption/ionization (MALDI) mass spectrometry an indispensable tool for biomolecular analysis.

Conventional, empirically selected matrices produce abundant matrix ion clusters in the low-mass region. Gold nanoparticles (AuNPs) assisted laser desorption/ionization mass spectrometry (GALDI-MS) provided new horizons and offered many functions for various applications.

This review summarized AuNPs applications for analytical, biotechnology and proteomics. AuNPs efficiently absorbed the laser radiation and transferred the energy to the analyte for the desorption/ionization process. Here, we describe a matrix-assisted laser desorption/ionization mass spectrometry imaging method for the imaging of peptides in Medicago truncatula root nodules.

Sample preparation steps including embedding in gelatin, sectioning, and matrix application are described.

INTRODUCTION. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced in clinical microbiology laboratories for identifying microorganisms (1 – 4).Although the initial investment in a mass spectrometer is relatively high, the cost of identifying a single isolate is lower than with previously used biochemical or molecular.

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry provides a new and powerful tool to analyse the distribution of metabolites within plant tissues. The two matrices alpha-cyanohydroxycinnamic acid (alpha-CHCA) and 9-aminoacridine provide a useful combination that allows the measurement of amino acids, sugars, and.

Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas. Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

This thesis presents efforts to improve the methodology of matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) as a method for analysis of metabolites from plant tissue samples.

The first chapter consists of a general introduction to the technique of MALDI-MSI, and the sixth and final chapter provides a brief summary. and matrices for matrix assisted laser desorption/ionization mass spectrometry.

Application of ionic liquids for MALDI-MS. Ionic liquids were used as effective matrices for MALDI-MS. They were applied for wide number of analytes such as lipids [49,54], proteins [55], peptides [7], sterols [54], oligonucleotides [56,57], polymers. A New method for analysis of disulfide-containing proteins by matrix-assisted laser desorption ionization (MALDI) mass spectrometry.

J Am Soc Mass Spectrom. ;20(12)– PubMed CrossRef Google Scholar. Adenosine -5′-triphosphate (ATP) plays a valuable role in metabolic activity to produce adequate energy in a biosystem.

A high ATP/AMP ratio has a correlation with diabetes that induces suppression of AMP-activated protein kinase (AMPK). Matrix-assisted laser desorption/ionization (MALDI)–mass spectrometry (MS) has outstanding potential in determining the ratio of several types of.Ghost peaks observed after atmospheric pressure matrix-assisted laser desorption/ionization experiments may disclose new ionization mechanism of matrix-assisted hypersonic velocity impact ionization.

Moskovets E. Moskovets E. Rapid Commun Mass Spectrom. Aug 30;29(16) doi: /rcmChemical derivatization coupled with matrix assisted laser desorption ionization time-of-flight mass spectrometry for convenient and efficient N-glycan detection in human plasma efficient detection of these disease-associated aberrations is of great significance for the development of new diagnostic biomarkers and therapeutic drug targets.